Mouse Anti-Human TIE-2

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Cat-Nr.101-M55
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#21G7)
IsotypeIgG1
Species ReactivityHuman
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human extracellular domain of TIE-2 fragment). The IgG1 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Antigenhuman Tie2 extracellular domain
ApplicationELISA, WB, IHC
SynonymsTEK; TIE2; VMCM; TIE-2; VMCM1; CD202B
DescriptionTie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2. Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.
Uniprot IDQ02763
Protein RefSeqNP_000450.2
mRNA RefSeqNM_000459.3

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