Rat Anti-Human FGFR-4

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Size100 µg
Price380 €
CategoryMonoclonal Antibody
Clone Nr.(#8F25)
Species ReactivityHuman
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThe antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with the recombinant extracellular domain of human Fibroblast Growth Factor Receptor-4 (sFGFR-4). The IgG2 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
AntigenHuman recombinant FGF-R4 EC domain
ApplicationWB, IHC, FC
SynonymsFGFR4; TKF; JTK2; CD334
DescriptionFibroblast growth factor receptor 4 (FGF R4), also known as CD334, is a 110 kDa glycosylated transmembrane receptor tyrosine kinase. Mature human FGF R4 consists of a 348 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 412 aa cytoplasmic domain that contains the tyrosine kinase domain. Within the ECD, human FGF R4 shares 90% and 88% aa sequence identity with mouse and rat FGF R4, respectively. Alternate splicing generates a potentially secreted isoform with a substitution encompassing the transmembrane segment. A 65 kDa N-terminally truncated isoform lacks the signal peptide and first two Ig-like domains. This isoform is produced in human pituitary adenomas and is constitutively phosphorylated and oncogenic. FGF R4 is widely expressed during embryonic development and in adult liver, kidney, and lung. It binds FGF acidic, FGF basic, FGF8, 15, and 19. FGF R4 associates with betaKlotho and sulfated glycosaminoglycans, and these interactions increase the affinity of FGF R4 for its ligands as well as its signaling capacity. FGF19 induced signaling through FGF R4 is important for the regulation of bile acid synthesis and lipid and glucose homeostasis. FGF R4 supports glucose tolerance and insulin sensitivity and protects against hyperlipidemia. It is downregulated in the liver during fasting and is upregulated by insulin. It can exert either proliferative or apoptotic effects on hepatocytes. FGF R4 signaling is additionally required for skeletal muscle development in limbs. FGF R4 interacts in cis with cell surface MMP14, leading to downregulation of both proteins.
Uniprot IDP22455
Protein RefSeqNP_002002
mRNA RefSeqNM_002011

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