Rat Anti-Mouse EphA4

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Cat-Nr.103-M166
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#11C13)
IsotypeIgG1
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with recombinant mouse EphA4 extracellular domain. IgG1 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
AntigenRecombinant mouse EphA4 extracellular domain
ApplicationWB, IHC (P)
SynonymsEpha4; rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584; 2900005C20Rik
DescriptionEphA4, also known as Sek, Sek1, Cek8, Hek8, and Tyro1, is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA4 has been shown to bind ephrinA5, ephrinA1, ephrinA3, ephrinA2, ephrinB2, ephrinB3, and ephrinA4. The extracellular domains of mouse and human EphA4 share greater than 95% amino acid identity. Only membranebound or Fcclustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation. In vivo, the ligands and receptors display reciprocal expression. It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue. The Eph/ephrin families also appear to play a role in angiogenesis.
Uniprot IDQ03137
Protein RefSeqNP_031962.2
mRNA RefSeqNM_007936.3

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