Mouse Anti-Human CCL4 (MIP-1 beta)

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Cat-Nr.101-M189
Size100 µg
Price350 €
CategoryMonoclonal Antibody
Clone Nr.(#12D21)
IsotypeIgG2
Species ReactivityHuman
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with human recombinant protein of CCL-4.
Antigen Recombinant Human CCL4
ApplicationWB, ICC, FC, N, Capture Ab
SynonymsMacrophage Inflammatory Protein-1β, CCL4, ACT-3
DescriptionCCL4, also known as macrophage inflammatory protein 1 beta (MIP1β), is a 12 kDa β chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP1α. The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV. Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of Mtropic HIV1 which utilizes CCR5 as a coreceptor. Both forms of CCL4 block HIV1 infection of T cells by inducing the downregulation of CCR5. Mature mouse CCL4 shares 77% and 86% amino acid sequence identity with human and rat CCL4, respectively.
Uniprot IDP13236
Protein RefSeqNP_002975.1
mRNA RefSeqNM_002984.3

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