Rat Anti-Mouse Indian Hedgehog

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Cat-Nr.103-M286
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#3A11)
IsotypeIgG2
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant protein of N-Terminal fragment of ihh. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
AntigenMouse recombinant protein of N-Terminal fragment of ihh protein
ApplicationWB
SynonymsIHH; BDA1; HHG2
DescriptionIndian Hedgehog (Ihh), Sonic Hedgehog (Shh), and Desert Hedgehog (Dhh) are important signaling molecules during embryonic development and are highly conserved within and across species. Mouse Ihh cDNA encodes a 411 amino acid (aa) polypeptide with a predicted 27 aa signal peptide. Post-translational processing yields a 19 kDa lipid-modified N-terminal domain that is the signaling molecule. At the cell surface, Ihh activity is mediated by binding the 12-pass transmembrane receptor, Patched (Ptc), and signaling through the 7-pass transmembrane G-protein coupled receptor, Smoothened (Smo).
Uniprot IDP97812
Protein RefSeqNP_034674.1
mRNA RefSeqNM_010544.2

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