Mouse Anti-Human VEGFR-1/Flt-1
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| Cat-Nr. | 101-M24 |
| Size | 100 µg |
| Price | 275 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#EWC) |
| Isotype | IgG1 |
| Species Reactivity | Human, mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular Flt-1 (D5) as the immunizing antigen. |
| Antigen | Recombinant human soluble Flt-1 (D5) |
| Application | ELISA, WB, IF |
| Synonyms | vascular endothelial growth factor receptor-1; FLT1; VEGF receptor 1 |
| Description | VEGFR-1 is a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia. |
| Uniprot ID | P17948 |
| Protein RefSeq | NP_001153392 |
| mRNA RefSeq | NM_001159920 |
Figures
IF staining of human Flt-1 in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with a mouse anti-human Flt-1 antibody [#101-M24].
Conjugated secondary antibody: goat anti-mouse ALEXA Flour (1:600) [Dianova].
Western analysis of recombinant human and mouse soluble VEGF receptors using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is a cross reactivity with mouse sFlt-1(D7)-Fc but no with human and mouse sKDR and human sFLT-4 visible.
Western analysis of recombinant mouse soluble VEGFR-1 conditioned supernatant using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is a strong cross reactivity with the mouse sFlt-1 visible.
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