Mouse Anti-Human Activin R2A

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Cat-Nr.101-M171
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#6J16)
IsotypeIgG2
Species ReactivityHuman
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with recombinant human Activin receptor IIA extra cellular domain. IgG2 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Antigenrecombinant human Activin receptor IIA extracellular domain
ApplicationWB, IHC
SynonymsACVR2A; ACVR2; ACTRII
DescriptionActivin proteins are involved in a wide range of biological processes including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, the regulation of reproductive physiology, inflammation, and carcinogenesis. They function through heteromeric complexes of type I and type II serine/threonine kinase receptors. Dimeric ligands bind to a type II receptor, such as Activin Receptor IIA (ActRIIA or activin R2A), which then associates with a type I receptor to initiate signal transduction. ActRIIA mediates the pleiotropic effects of Activins and Inhibins as well as several members of the BMP and GDF families of TGF β like proteins. Mature human ActRIIA is a 70 kDa glycoprotein that consists of a 116 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 352 aa cytoplasmic region that includes the kinase domain and a PDZbinding motif. Within the ECD, human ActRIIA shares 98% aa sequence identity with mouse and rat ActRIIA. Signaling through ActRIIA is modulated by its interaction with RGMB/ DRAGON, Cripto, Endoglin/CD105, TGFβ RIII/Betaglycan, or BAMBI. These interactions can enhance ligandinduced signaling or interfere with signaling by preventing ActRIIA association with type I receptors. Activininduced responses can also be limited by the enhanced internalization of ActRIIA following its association with the cytoplasmic proteins ARIP1 and ARIP2.
Uniprot IDP27037
Protein RefSeqNP_001607.1
mRNA RefSeqNM_001616.3

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