Mouse Anti-Human Orexin B
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|Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
|Stability and Storage
|Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.
|This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with extracellular domain of human recombinant protein of Orexin-B.
|human Orexin-B peptide (aa71-96, O43621)
|Orexin B is a hypothalamic neuropeptide that regulates feeding behavior. This peptide significantly augments food intake in a dose-dependent manner when a bolus was administered intracerebroventricularly. After a 48 hour fast, hypothalamic prepro-orexin mRNA level is up-regulated 2.4. The discovery of Orexins and their receptors may provide a novel molecular basis for understanding the role of the lateral hypothalamic areas in the regulation of energy homeostasis.
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