Mouse Anti-Human Ephrin-B3

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Size100 µg
Price380 €
CategoryMonoclonal Antibody
Clone Nr.(#12X1)
Species ReactivityHuman
ReconstitutionCentrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant Ephrin-B3 extracellular domain.
Antigenrecombinant human Ephrin-B3 EC domain
SynonymsEFNB3; EFL6; EPLG8; LERK8
DescriptionEphrinB3, also known as NLERK2, ElkL3, EFL6, ELF3 and LERK8, is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids (aa) and includes four invariant cysteines. The B class ligands are transmembrane proteins which can be tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 aa long and are highly conserved, especially the last 33 aa. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. EphrinB3 has been shown to bind EphrinA4, EphrinB1, EphrinB2, and EphrinB3. The extracellular domains of murine and human EphrinB3 share 98% aa identity. Only membranebound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation. In vivo, the ligands and receptors display reciprocal expression. It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue. The Ephrin/Eph families also appear to play a role in angiogenesis.
Uniprot IDQ15768
Protein RefSeqNP_001397.1
mRNA RefSeqNM_001406.3

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