rat Anti-Mouse MMRN-2
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| Cat-Nr. | 103-M512 |
| Size | 100 µg |
| Price | 275 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#2063E2A11) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | recombinant mouse Multimerin-2 |
| Application | WB, IF, IHC, ELISA |
| Synonyms | Multimerin-2, MMRN2 |
| Description | MMRN2 is an extracellular matrix (ECM) glycoprotein named Multimerin2 (MMRN2) previously known as EndoGlyx-1, which has been shown to be specifically expressed in endothelial cells of vascular origin. MMRN2 is found along the blood vessels in close contact with endothelial cells in normal and tumor vasculature and contributes to the maintenance of blood vessels’ homeostasis by affecting the VEGF-A/VEGFR2 axis (Lorenzon et al., 2012). Neither proliferation nor apoptotic rate are affected. By preventing the interaction of VEGF-A with VEGFR1 and VEGFR2, MMRN2 reduces their activation and impaires the non-integrin dependent motility of endothelial cells. It is conceivable that MMRN2 attains a high endothelial cells pericellular concentration and thus competes for the binding of VEGF-A to its receptors. MMRN2 may thus represent a novel key homeostatic molecule indispensable for the maintenance of blood vessel integrity. This antibody has been successfully used in detection ELISA, immunohistochemistry and immunoblot assays. MMRN2 is highly specific for endothelium and stains all endothelial cells. |
| Uniprot ID | A6H6E2 |
| Protein RefSeq | NP_694767.3. |
| mRNA RefSeq | NM_153127.3. |
Figures
Labeling of mouse brain endothelial cells (bEnd) with a rat anti-mouse Multimerin-2 (MMRN2) monoclonal antibody [Cat# 103-512].
Cryostat section of mouse skin papilloma. In red lymphatic vessels stained with anti LYVE-1; in green blood vessels stained with anti MMRN2 (clone 2063E2A11) [Cat# 103-M512]; in blue, nuclear staining. Scale bar: 300 μm
Cryostat section of mouse lymph node. The lymphatic vessels positive for LYVE-1 are stained in red, the blood vessels positive for MMRN2 are stained in green. Scale bar: 300 μm
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