Mouse Anti-Human Emilin-1
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| Cat-Nr. | 101-M09 |
| Size | 100 µg |
| Price | 275 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#1H2/G8) |
| Isotype | IgG2a |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | recombinant human Emilin-1 |
| Application | WB, IF, IHC, ELISA |
| Synonyms | Elastin microfibril interface-located protein 1, Elastin microfibril interfacer 1, EMI |
| Description | Emilins (elastin microfibril interface located proteins) are extracellular matrix glycoproteins that localize to sites with proximity to elastin and microfibrils. They consist of an N-terminal cysteine-rich EMI domain and a conserved C-terminal gC1q-like domain. Emilin-1 is abundant in elastin-rich tissues such as blood vessels, skin, heart and lung. It influences placenta formation and initial organogenesis with a later role in interstitial connective tissue. Emilin-2 is larger than Emilin-1 and contains a unique proline-rich domain. It is likely involved in the process of elastogenesis. Multimerin-2 (also known as Emilin-3 or EndoGlyx-1) is expressed during embryonic development. Multimerin-1 (also known as Emilin-4) is expressed in platelets and the endothelium of blood vessels and may act as a carrier protein for platelet factor V. Emilin-5 is encoded by the Emilin-3 gene and is sometimes referred to as Emilin-3. It contains the N-terminal cysteine-rich EMI domain but lacks the C-terminal gC1q-like domain. Emilin-1 have been shown to be expressed in smooth muscle and other mesenchymal tissues and is localized at the proximity of elastin and microfibrils. Emilin-1 exerts an important role in lymphatic system, being a crucial structural molecule that regulates the formation of lymphatic capillaries and collectors. Emilin-1 through the interaction with the α4β1 integrin via the C-terminal gC1q domain exerts a negative effect on proliferation. It binds pro-TGFβ preventing its maturation to mature TGFβ in the extracellular space, therefore influencing the regulation of blood and lymph vessels formation and maintenance. |
| Uniprot ID | Q9Y6C2 |
| Protein RefSeq | NP_008977 |
| mRNA RefSeq | NM_007046.3 |
Figures
Western blot analysis with anti-human EMILIN1 #1H2/G8 antibody [Cat# 101-M09] on recombinant human EMILIN1 purified from HEK293 transfected cells.
Function Blocking Activity: Schematic diagram depicting the epitope localization of three clones of anti EMILIN mAbs (from Spessotto et al., 2003). Bottom, left: cell adhesion to EMILIN1 in the presence of anti EMILIN1 mAbs (adapted from Spessotto et al., 2003). Bottom right: levels of cell adhesion to EMILIN1 gC1q domain in the presence of the anti-EMILIN1 mAb #1H2/G8 [Cat# 101-M09] or the anti-α4 integrin subunit mAb P1H4 (adapted from Spessotto et al., 2006).
Functional ELISA: Supernatant (200μl) and total cell lysate (100μl, 1:2 diluted) of HEK293 cells expressing the different EMILIN proteins were coated to a 96-well plate and incubated at 37°C for >1 hour. After blocking and several wash steps the #1H2/G8 [Cat# 101-M09] antibody was added (2μg/ml) and incubated for another 1 hour. Detection was performed with an anti-mouse secondary antibody.
Immunofluorescence staining of normal cultured cells (skin human fibroblasts) (panel a, left) and of cryostat tissue section (undifferentiated soft tissue sarcoma) (panel b, right) with #1H2/G8 [Cat# 101-M09]) (green staining). Nuclei are in blue.
Dotted lines indicate the border between “normal” (lower corner) and “tumor” tissue.
Immunofluorescence staining of human intestine cryostat sections with the monoclonal mouse anti-human EMILIN1 antibody #1H2/G8 [Cat# 101-M09].
Immunofluorescence staining of human EMILIN1 in HEK293 cells expressing human EMILIN1 with the monoclonal mouse anti-human EMILIN1 antibody #1H2/G8 [Cat# 101-M09]. Conjugated secondary antibody: goat anti-mouse ALEXA Flour 488 (1:600) [Dianova].
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