Rat Anti-Mouse CD25

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Cat-Nr.103-M107
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#7G19)
IsotypeIgG1
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 400 µl sterile PBS and the final concentration is 500 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse CD25 protein). IgG1 fraction of the culture supernatant was purified by Protein G affinity chromatography.
AntigenRecombinant Mouse CD25 protein
ApplicationIHC, FC
SynonymsCD25; Il2r; Ly-43; interleukin 2 receptor, alpha chain; Il2ra
DescriptionThe biological effects of interleukin 2 (IL-2) are mediated by its binding to a multi-chain cellular receptor complex. The IL-2 receptor (IL-2 R) complex consists of three glycoprotein chains. The alpha chain (IL-2 R alpha /CD25) and beta chain (IL-2 R beta /CD122) act together with the gamma chain (IL-2 R gamma /CD132) to form a high affinity receptor that transduces the signal upon IL-2 binding. Alone, IL-2 R alpha binds IL-2 with low affinity, but is unable to transduce a signal. The alpha/beta combination binds IL-2 with intermediate affinity, but is still unable to transmit a signal inside the cell. The beta/gamma complex has intermediate affinity and is capable of signaling if the concentration of IL-2 is relatively high.
Uniprot IDP01590
Protein RefSeqNP_032393.3
mRNA RefSeqNM_008367.3

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