Mouse Anti-Human TIE-2
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| Cat-Nr. | 101-M50 |
| Size | 100 µg |
| Price | 240 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#tek2) |
| Isotype | IgG1 |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular TIE-2 as the immunizing antigen. Mouse IgG1 antibody (#tek2) from hybridomas was purified from cell culture supernatant by Protein G chromatography. |
| Antigen | human soluble extracellular TIE-2 |
| Application | ELISA, WB, FC |
| Synonyms | TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B |
| Description | Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2. Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects. |
| Uniprot ID | Q02763 |
| Protein RefSeq | NP_000450.2 |
| mRNA RefSeq | NM_000459.3 |
Figures
IHC with cryo sections of human spleen.
The experiment was performed by Prof. Dr. Birte Steiniger, Institute of Anatomy and Cell Biology in Marburg, Germany
Quantification of soluble and cellular TIE-2 by sandwich ELISA.
CM and cell lysates from HUVECs treated with PMA (25ng/ml) or left untreated were analysed by Sandwich ELISA for the concentrations of sTIE-2 or TIE-2. For capturing anti-human TIE-2 Cl.16 [#101-M54] was used, for the detection a mixture of biotinylated anti-human TIE-2 Cl.2 [#101-M50] and Cl.9 [#101-M52].
FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC).
Immunofluorescence staining (green) of cryo-sections of human placenta tissue (fixed shortly in 4% PFA) (A) with anti-human TIE2 #tek2 [0.5mg/ml, 1:100] (Cat# 101-M50) and (B) control without primary antibody [20x objective]. Signal is mainly found in endothelial cells of the placenta.
The experiment was performed by the research group of Prof. Dr. J. Wilting, University Göttingen, Germany.
Effects of PMA treatment on TIE-2.
HUVECs (passage 1) were stimulated for the indicated periods of time with PMA at 25 ng/ml or left untreated. Western blot analysis for the presence of TIE-2 protein by immunoprecipitation using antibodies directed against the extracellular domain of human TIE-2 (IP: TEK16 [#101-M54]; Western: TEK2 [#101-M50]).
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