Mouse Anti-Human TIE-2
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Cat-Nr. | 101-M50 |
Size | 100 µg |
Price | 255 € |
Category | Monoclonal Antibody |
Clone Nr. | (#tek2) |
Isotype | IgG1 |
Species Reactivity | Human |
Formulation | lyophilized |
Buffer | PBS |
Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
Preparation | The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular TIE-2 as the immunizing antigen. Mouse IgG1 antibody (#tek2) from hybridomas was purified from cell culture supernatant by Protein G chromatography. |
Antigen | human soluble extracellular TIE-2 |
Application | ELISA, WB, FC |
Synonyms | TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B |
Description | Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2. Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects. |
Uniprot ID | Q02763 |
Protein RefSeq | NP_000450.2 |
mRNA RefSeq | NM_000459.3 |
Figures
![](/fileadmin/user_upload/products/TIE/101-M50_IHC.jpg)
IHC with cryo sections of human spleen.
The experiment was performed by Prof. Dr. Birte Steiniger, Institute of Anatomy and Cell Biology in Marburg, Germany
![](/fileadmin/user_upload/products/TIE/101-M50_52_54_ELISA.jpg)
Quantification of soluble and cellular TIE-2 by sandwich ELISA.
CM and cell lysates from HUVECs treated with PMA (25ng/ml) or left untreated were analysed by Sandwich ELISA for the concentrations of sTIE-2 or TIE-2. For capturing anti-human TIE-2 Cl.16 [#101-M54] was used, for the detection a mixture of biotinylated anti-human TIE-2 Cl.2 [#101-M50] and Cl.9 [#101-M52].
![](/fileadmin/user_upload/products/TIE/101-M50_FC_4.jpg)
FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC).
![](/fileadmin/user_upload/products/TIE/101-M50_a-TIE2__tek2_IHC.jpg)
Immunofluorescence staining (green) of cryo-sections of human placenta tissue (fixed shortly in 4% PFA) (A) with anti-human TIE2 #tek2 [0.5mg/ml, 1:100] (Cat# 101-M50) and (B) control without primary antibody [20x objective]. Signal is mainly found in endothelial cells of the placenta.
The experiment was performed by the research group of Prof. Dr. J. Wilting, University Göttingen, Germany.
![](/fileadmin/user_upload/products/TIE/101-M54_Blot_IP.jpg)
Effects of PMA treatment on TIE-2.
HUVECs (passage 1) were stimulated for the indicated periods of time with PMA at 25 ng/ml or left untreated. Western blot analysis for the presence of TIE-2 protein by immunoprecipitation using antibodies directed against the extracellular domain of human TIE-2 (IP: TEK16 [#101-M54]; Western: TEK2 [#101-M50]).
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