Rat Anti-Mouse 4-1BBL

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Cat-Nr.103-M209
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#MAB0875)
IsotypeIgG
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant 4-1BB Ligand extracellular domain. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
AntigenBALB/c mouse B lymphoma cell line
ApplicationFC, Blocking
SynonymsTnfsf9; Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817; CDw137L
Description4-1BB ligand (4-1BBL) is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF9. Mouse 4-1BBL cDNA encodes a 309 amino acid residues (aa) protein with an 82 aa Nterminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C terminal extracellular domain. The extracellular domain of 4-1BBL has a tertiary structure similar to that of other TNFSF members, but shares only low aa sequence homology (14-16%). Murine 4-1BBL shares 36% aa sequence identity with its human counterpart. 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines. A soluble 4-1BBL is released from the cell surface following cellular activation via proteolytic cleavage by one or more sheddases. By analogy to other TNFSF ligands, both the soluble and transmembrane 4-1BBL are expected to exist as noncovalent homotrimers. 4-1BBL binds 4-1BB, a TNF receptor superfamily member, TNFRSF9, which is also known as CD137 and ILA (induced by lymphocyte activation). 41BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils. In response to 4-1BBL binding, 4-1BB transduces a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In dendritic cells, 4-1BB is a DC-activating molecule that enhances cytokine production and upregulates expression of B71 and B72 costimulatory molecules.
Uniprot IDP41274
Protein RefSeqNP_033430.1
mRNA RefSeqNM_009404.3

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