Mouse Anti-Rat Podoplanin
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| Cat-Nr. | 104-M40 |
| Size | 100 µg |
| Price | 260 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#LF3(B7)D5B3) |
| Isotype | IgG |
| Species Reactivity | Rat |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Monoclonal antibodies were produced with the help of BALB/c mice using a membrane protein fraction of isolated rat glomeruli as the immunizing antigen. The mouse IgG antibody (#LF3(B7)D5B3) from hybridomas was purified from cell culture supernatant by Protein G chromatography. |
| Antigen | membrane protein fraction of isolated rat glomeruli |
| Application | WB, IHC |
| Synonyms | Pdpn; E11; Gp38; OTS-8; RTI40; T1-alpha |
| Description | Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. It was originally discovered in kidney podocytes. This mouse monoclonal antibody (IgG) against rat podoplanin works well for immunohistochemistry with formalin-fixed paraffin embedded sections. This antibody also can be used in FACS sorting. |
| Uniprot ID | Q64294 |
| Protein RefSeq | NP_062231.1 |
| mRNA RefSeq | NM_019358.1 |
Figures
Staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle (paraffin-embedded tissue-sections fixed in formalin) with anti-rat Podoplanin [Cat# 104-M40].
Immunofluorescence staining of lymphatic endothelial cells and podocytes in normal rat renal corpusle (paraffin-embedded tissue-sections fixed in formalin) with anti-rat Podoplanin [Cat# 104-M40].
Rat cardiac lymphatic microvessels, labeled with a monoclonal antibody against rat Podoplanin (green) [Cat# 104-M40, left panel] and a polyclonal antibody against mouse LYVE-1 [Cat# 103-PA50; right panel] (red). Image was obtained at 20x magnification on a Zeiss fluorescence microscope. Scale bar = 50 μm. The used protocol in short was: 1. Blockage of nonspecific binding; 2. Incubation with primary abs : anti-mouse Lyve1 (1:1000) and mouse anti-Podoplanin (1:400) for 60 min at RT; 3. Incubation with secondary abs: Donkey anti-rabbit Cy3 and Donkey anti-mouse FITC, 30 min at RT; 4. Mounting in DAPI-containing medium for cell nuclei labeling.
The experiments were performed by the research group of Prof. Dr. E. Brakenhielm – Rouen University (see also: Henri O et al., Circulation, March 2016, DOI: 10.1161).
Western blot analysis with recombinant human, mouse and rat soluble Podoplanin. There is no cross reaction with human and mouse Podoplanin.
Reference
- ESWT Diminishes Axonal Regeneration following Repair of the Rat Median Nerve with Muscle-In-Vein Conduits but Not after Autologous Nerve Grafting. J. C. Heinzel et al., Biomedicines. 2022 Aug; 10(8): 1777.
- Occurrence of Lymphangiogenesis in Peripheral Nerve Autografts Contrasts Schwann Cell-Induced Apoptosis of Lymphatic Endothelial Cells In Vitro. C. Hromada et al., Biomolecules. 2022 Jun; 12(6): 820.
- Selective Stimulation of Cardiac Lymphangiogenesis Reduces Myocardial Edema and Fibrosis Leading to Improved Cardiac Function Following Myocardial Infarction. O. Henri et al., Circulation. 2016 Apr 12;133(15):1484-97.
- Insufficient Lymph Drainage Causes Abnormal Lipid Accumulation and Vein Wall Degeneration. Hiroki Tanaka et al., Ann Vasc Dis. 2016; 9(4): 277–284.
- Filarial Excretory-Secretory Products Induce Human Monocytes to Produce Lymphangiogenic Mediators. T. Weinkopff et al., PLoS Negl Trop Dis. 2014 Jul; 8(7): e2893.
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