Mouse Anti-Rat Podoplanin

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Cat-Nr.104-M40
Size100 µg
Price230 €
CategoryMonoclonal Antibody
Clone Nr.(#LF3(B7)D5B3)
IsotypeIgG
Species ReactivityRat
Formulationlyophilized
BufferPBS
ReconstitutionCentrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Stability and StorageThe lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.
PreparationMonoclonal antibodies were produced with the help of BALB/c mice using a membrane protein fraction of isolated rat glomeruli as the immunizing antigen. The mouse IgG antibody (#LF3(B7)D5B3) from hybridomas was purified from cell culture supernatant by Protein G chromatography.
Antigenmembrane protein fraction of isolated rat glomeruli
ApplicationWB, IHC
SynonymsPdpn; E11; Gp38; OTS-8; RTI40; T1-alpha
DescriptionPodoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. It was originally discovered in kidney podocytes. This mouse monoclonal antibody (IgG) against rat podoplanin works well for immunohistochemistry with formalin-fixed paraffin embedded sections. This antibody also can be used in FACS sorting.
Uniprot IDQ64294
Protein RefSeqNP_062231.1
mRNA RefSeqNM_019358.1

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Reference

  1. Insufficient Lymph Drainage Causes Abnormal Lipid Accumulation and Vein Wall Degeneration. Hiroki Tanaka et al., Ann Vasc Dis. 2016; 9(4): 277–284.
  2. Filarial Excretory-Secretory Products Induce Human Monocytes to Produce Lymphangiogenic Mediators. T. Weinkopff et al., PLoS Negl Trop Dis. 2014 Jul; 8(7): e2893.

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