Rat Anti-Mouse HGF Activator Inhibitor Type 1 (HAI-1)

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Cat-Nr.103-M229
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#3G21)
IsotypeIgG2
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant protein of HAI-1extracellular domain. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
Antigenrecombinant mouse HAI-1 extracellular domain
ApplicationWB
SynonymsSpint1; HAI-1
DescriptionHepatocyte growth factor activator inhibitor1 (HAI1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGF-A) and matripas. The membraneanchored HAI1 consists of two Kunitz domains, a LDL-recepto-rlike domain, and a C-terminal transmembrane domain. Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface. The shedding of HAI1 and HGFA/HAI1 complex is enhanced by treatment with phorbol 12myristate, 13acetate or IL1β. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor.
Uniprot IDQ9R097
Protein RefSeqNP_058603.2
mRNA RefSeqNM_016907.3

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