Rat Anti-Mouse Emilin-1
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| Cat-Nr. | 103-M80 |
| Size | 100 µg |
| Price | 250 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#C11A8) |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Antigen | recombinant mouse Emilin-1 |
| Application | WB, IHC (F) |
| Synonyms | gp115; AW229038; BB105748; EMILIN-1; 5830419M17Rik; elastin microfibril interface located protein 1 |
| Description | Emilin-1 is an extracellular matrix glycoprotein localized at sites where elastin and microfibrils are in proximity. It may be responsible for anchoring smooth muscle cells to elastic fibers. It has cell adhesive capacity. Emilin-1 may have a role in the regulation of blood vessel assembly since it inhibits TGFB signaling by binding specifically to the pro-TGFB precursor and preventing its maturation by furin convertases in the extracellular space. TGFB proteins are the main regulators of blood vessel development and maintenance. |
| Uniprot ID | Q99K41 |
| Protein RefSeq | NP_598679.1 |
| mRNA RefSeq | NM_133918.2 |
Figures
Western blot on recombinant protein obtained from supernatant of cells expressing human EMILIN1 (hE1), mouse EMILIN1 (mE1), mouse EMILIN2 (mE2). There is no cross reactivity with human Emilin1 an mouse Emilin2.
Positive staining for EMILIN1 (green) in mouse lung tissue is both detected in lung parenchymal fibers and associated with lymphatic vessel structures. Lyve-1 (red) is used as lymphatic vessel marker. Blue, nuclei. Scale bar: 37.00 μm
Positive staining for EMILIN1 (red) in mouse skin. Blue, nuclei; Scale bar: 37.00 μm
Positive staining of EMILIN1 (green) in mouse lymphatic endothelial cells (LAEC). Nuclei are stained in blue. Scale bar 38 μm.
Positive staining for EMILIN1 (green) in mouse lymph node. In red, lymphatic vessels positive for Lyve-1. Yellow represents the close association of EMILIN1 with lymphatic vessel structures. Scale bar: 37.00 μm
Positive staining of EMILIN1 (green) in mouse fibroblasts (NIH 3T3). Actin cytoskeleton is stained in red, nuclei in blue. Scale bar 28 μm.
EMILIN1 is expressed in association with lymphatic vessels. Cryostat sections of normal mouse tissues stained with anti-EMILIN1 (green) antibodies [Cat# 103-M80]. In all mouse tissues and organs examined, EMILIN1 was uniformly distributed in the stroma. In the skin, EMILIN1 staining colocalizes with LYVE-1-positive lymphatic vessels surrounding hair follicles. In the small intestine, EMILIN1 colocalizes with LYVE-1-positive lacteals and submucosal lymphatic vessels. At higher magnification, in the lung and lymph nodes, it is more evident that EMILIN1 is distributed at the abluminal surfaces of LECs . In the lymph node, EMILIN1-positive fibers connecting LECs to the surrounding ECM are evident.
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