Rat Anti-Mouse LEC26
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| Cat-Nr. | 103-M152 |
| Size | 100 µg |
| Price | 260 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#LA102) |
| Isotype | IgG2b |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | The antibody was produced by a rapid differential immunization of DA rats with collagenase- and neuraminidase-treated mouse benign lymphangiomas. The rat IgG2b antibody was purified from cell culture supernatant by Protein G chromatography. |
| Antigen | with collagenase- and neuraminidase-treated mouse benign lymphangiomas |
| Application | WB, IHC (C), IF |
| Synonyms | Lymphatic endothelial cell marker 26 |
| Description | LA102 reacts with mouse lymphatic vessels except the thoracic duct and the marginal sinus of lymph nodes, but not with any blood vessels. LA102 recognized a protein of around 26 kDa. The antigen recognized by LA102 was localized on both luminal and abluminal lymphatic endothelial cell membranes. Besides lymphatics, some lymphoid cells are also recognized. |
Figures
Specificity: LA102 [Cat# 103-M152] reacts with mouse lymphatic vessels except the thoracic duct and the marginal sinus of lymph nodes, but not with any blood vessels. LA102 recognized a protein of around 26 kDa. The antigen recognized by LA102 was localized on both luminal and abluminal lymphatic endothelial cell membranes. Besides lymphatics, some lymphoid cells are also recognized.
The experiments were performed by the research group of Prof. T. Ezaki, TWMU, Tokyo, Japan.
Immunofluorescence staining of BEC12/LA5 (green) [Cat# 103-M154] and LEC26/LA102 (red) [Cat# 103-M152] in small intestine from C57BL/6 female mice. Fresh frozen cryosections (10 micron thick) were fixed with acetone for 10 min at RT and then reacted directly with Biotin-conjugated LA102 followed by streptoavidin-Cy3 (red) and Alexa488 labeled LA5 (green) for double staining.
The experiments were performed by the research group of Prof. T. Ezaki, TWMU, Tokyo, Japan.
Double staining of mouse tongue with LA102 [Cat# 103-M152] (Alexa546: red) and FITC (green)-conjugated tomato-lectin (LEL).
The experiments were performed by the research group of Prof. T. Ezaki, TWMU, Tokyo, Japan.
Immunofluorescence staining of LEC26 (#LA102) in primary mouse endothelial cells (mEC) with anti-mouse LEC26 (10µg/ml) [Cat# 103-M152] and counter staining of nuclei with Dapi. As secondary antibody goat anti-rat ALEXA Flour 488 (Dianova) was used 1:400.
Immunoenzymatic staining of mouse spleen cryosection (fixed with 4% paraformaldehyde in PBS). Lymphatic vessels in the trabecula area are strongly stained with LA102 [Cat# 103-M152] (colored blue by ALP-Vector blue reaction). Some strongly stained lymphoid cells are also seen in the red pulp.
The experiments were performed by the research group of Prof. T. Ezaki, TWMU, Tokyo, Japan.
Immunoenzymatic staining of mouse spleen with LA102 [Cat# 103-M152]. Cells in the PALS (periarterial lymphoid sheath = mainly T cell area) are well stained with LA102 (colored brown by HRP-DAB reaction).
The experiments were performed by the research group of Prof. T. Ezaki, TWMU, Tokyo, Japan.
Reference
- Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels. J. Schniedermann et al., BMC Cell Biol. 2010; 11: 50.
- Methods to study lymphatic vessel integrins. B. Garmy-Susini et al., Methods Enzymol. 2007;426:415-38.
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