Rat Anti-Mouse LEC26

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Cat-Nr.103-M152
Size100 µg
Price275 €
CategoryMonoclonal Antibody
Clone Nr.(#LA102)
IsotypeIgG2b
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionCentrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Stability and StorageThe lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
PreparationThe antibody was produced by a rapid differential immunization of DA rats with collagenase- and neuraminidase-treated mouse benign lymphangiomas. The rat IgG2b antibody was purified from cell culture supernatant by Protein G chromatography.
Antigenwith collagenase- and neuraminidase-treated mouse benign lymphangiomas
ApplicationWB, IHC (C), IF
SynonymsLymphatic endothelial cell marker 26
DescriptionLA102 reacts with mouse lymphatic vessels except the thoracic duct and the marginal sinus of lymph nodes, but not with any blood vessels. LA102 recognized a protein of around 26 kDa. The antigen recognized by LA102 was localized on both luminal and abluminal lymphatic endothelial cell membranes. Besides lymphatics, some lymphoid cells are also recognized.

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Reference

  1. Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels. J. Schniedermann et al., BMC Cell Biol. 2010; 11: 50.
  2. Methods to study lymphatic vessel integrins. B. Garmy-Susini et al., Methods Enzymol. 2007;426:415-38.

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