Rat Anti-Mouse ICAM-1
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| Cat-Nr. | 103-M97 |
| Size | 100 µg |
| Price | 350 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#MAB0802) |
| Isotype | IgG2 |
| Species Reactivity | Mouse |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml. |
| Stability and Storage | Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity. |
| Preparation | This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with cytokine stimulated mouse endothelial cells. IgG fraction of the culture supernatant was purified by Protein A/G affinity chromatography. ICAM-1, a 95 kDa surface antigen found on endothelial cells, lymphocytes,epithelial cells, dendritic cells, and keratinocytes. ICAM-1 is a ligand for LFA-1 (CD11a/CD18) and MAC-1(CD11b/CD18). Its expression is up regulated upon stimulation by inflammatory mediators such as cytokines and LPS and it’s involved in B cell T-cell co- stimulatory interactions. |
| Antigen | Cytokine stimulated Mouse Endothelial cells |
| Application | IHC (P), FC |
| Synonyms | Icam1; CD54; Ly-47; Icam-1; MALA-2 |
| Description | Intercellular adhesion molecule 1 (ICAM-1), also known as CD54, binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface. |
| Uniprot ID | P13597 |
| Protein RefSeq | NP_034623.1 |
| mRNA RefSeq | NM_010493.2 |
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