Mouse Anti-Human Angiopoietin-4

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Cat-Nr.101-M216
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#11M11)
IsotypeIgG1
Species ReactivityHuman
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with Human Angiopoietin-4 recombinant protein.
Antigenrecombinant human Angiopoietin-4
ApplicationWB
SynonymsANGPTL4; NL2; ARP4; FIAF; PGAR; HFARP; pp1158; ANGPTL2
DescriptionHuman Angiopoietin4 (Ang4), alternatively named Ang3, is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiledcoiled domain near the aminoterminus and a fibrinogen like domain at the Cterminus. Human Ang 4 cDNA encodes a 503 amino acid (aa) precursor protein with a 23 aa signal peptide. It shares 45%, 47%, and 54% aa sequence identity with human Ang1, human Ang2, and mouse Ang3, respectively. Although the sequence homology is much higher between the human and mouse counterparts for Ang1 (97%) and Ang2 (85%), mouse Ang3 is believed to be an ortholog of human Ang4 based on chromosomal localization studies. Human Ang4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse Ang3 is expressed multiple mouse tissues. Human Ang4 is an agonist that can bind and activate Tie2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Mouse Ang3 has been reported to be a Tie2 antagonist. It is likely that mouse Ang3, like Ang2, may exert agonist or antagonist activities depending on the cell context.
Uniprot IDQ9Y264
Protein RefSeqNP_057069.1
mRNA RefSeqNM_015985.2

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