Mouse Anti-Human CEACAM-1
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| Cat-Nr. | 101-M182 |
| Size | 100 µg |
| Price | 260 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#B3) |
| Isotype | IgG1, kappa |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | Monoclonal antibodies were produced with the help of BALB/c mice using recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells) as the immunizing antigen. The antibody (clone #B3) was purified from cell culture supernatant by Protein G chromatography. |
| Antigen | recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells) |
| Application | WB, IHC, E, IF, IP, FC |
| Synonyms | CEACAM1; BGP; BGP1; BGPI, CD66a |
| Description | Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM-1), also known as CD66a and BGP-I, is a 115 kDa type I transmembrane glycoprotein that belongs to the membrane-bound CEA subfamily of the CEA superfamily. On the surface of cells, it forms noncovalent homo- and heterodimers. The extracellular region contains three C2-type Ig-like domains and one N-terminal V-type Ig-like domain. Multiple splice variants involving regions C-terminal to the second C2-type domain (aa 320 and beyond) exist. |
| Uniprot ID | P13688 |
| Protein RefSeq | NP_001192273.1 |
| mRNA RefSeq | NM_001205344.1 |
Figures
Flow cytometry with stably transfected HeLa cells: 250.000 Hela-human CEACAM1 cells; 10 µg/ml of primary B3-17 [Cat# 101-M182]. Binding was detected with a FITC-conjugated secondary antibody.
The experiments were performed by PD Dr. Bernhard Singer, Institute of Anatomy, Medical Faculty, University Duisburg-Essen, Germany.
IHC staining of human jejunum tissue with mAb B3 [Cat# 101-M182]. CEACAM1 was detected in PFA-fixed paraffin-embedded sections of human jejunum tissue using mAb B3 [Cat# 101-M182] followed by staining with antimouse HRP-DAB and counterstaining with hematoxylin. The labeling showed week staining of CEACAM1 by B3.
The experiments were performed by PD Dr. Bernhard Singer, Institute of Anatomy, Medical Faculty, University Duisburg-Essen, Germany.
Western blot analysis: Human CEACAM1 lysate
Detection utilizing 10 µg/ml [Cat# 101-M182]
The experiments were performed by PD Dr. Bernhard Singer, Institute of Anatomy, Medical Faculty, University Duisburg-Essen, Germany.
Sandwich ELISA: Solid phase was coated with 3 µg/ml anti-CEA (Dako) binding human CEACAM1. After washing, blocking and coating human CEACAM1 antigen, detecting antibody B3-17 [Cat# 101-M182] (10 µg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
NOTE: In Sandwich ELISA B3-17 [Cat# 101-M182] can also be used as catcher antibody.
The experiments were performed by PD Dr. Bernhard Singer, Institute of Anatomy, Medical Faculty, University Duisburg-Essen, Germany.
Immunofluorescence with HeLa-CEACAM1 cells. Cells were fixed with 4% PFA (10min) and then incubated in 5% BSA/PBS for 1h to block non-specific protein-protein interactions. The cells were then incubated with 10µg B3-17 [Cat# 101-M182] overnight at 4°C. The secondary antibody (green) was Alexa Flour® 488 goat-anti-mouse IgG (H+L).
The experiments were performed by PD Dr. Bernhard Singer, Institute of Anatomy, Medical Faculty, University Duisburg-Essen, Germany.
Reference
- Binding of Candida albicans to Human CEACAM1 and CEACAM6 Modulates the Inflammatory Response of Intestinal Epithelial Cells. E. Klaile et al., mBio. 2017 Mar-Apr; 8(2): e02142-16.
- CEACAM1 induces B-cell survival and is essential for protective antiviral antibody production. V. Khairnar et al., Nat Commun. 2015 Feb 18; 6: 6217.
- Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions. M. Seifert et al., Proc Natl Acad Sci U S A. 2015 Feb 10; 112(6): E546–E555.
- Soluble CEACAM8 Interacts with CEACAM1 Inhibiting TLR2-Triggered Immune Responses. B. B. Singer et al., PLoS One. 2014; 9(4): e94106.
- Tumor and Endothelial Cell-Derived Microvesicles Carry Distinct CEACAMs and Influence T-Cell Behavior. H. T. Muturi et al., PLoS One. 2013; 8(9): e74654.
- Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons. E. Klaile et al., Respir Res. 2013; 14(1): 85.
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