Rat Anti-Mouse Frizzled-8

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Cat-Nr.103-M389
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#11G21)
IsotypeIgG2
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of Frizzled 8.
Antigenrecombinant mouse protein Frizzled 8
ApplicationWB
SynonymsFzd8; Fz8
DescriptionWnt signaling is involved in a variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The founding member of this family was identified in Drosophilia based on its role in tissue polarity in the adult cuticle and named for the disorganized appearance of bristle hairs on the mutant. Mouse Frizzled8 (mFrz8) was originally cloned in a PCR screen to identify additional Frizzled family members in vertebrates. There are nine murine Frizzled genes and 10 human Frizzled genes identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a seven-pass transmembrane region, and a variablelength C-terminal tail. The most conserved regions of the Frizzled proteins are the extracellular cysteine rich domain (CRD) which spans approximately 120 amino acid residues and contains 10 invariant cysteines, and the seven transmembrane domains. Mouse Frizzled8 is 685 amino acid residues long and shows 95% and 82% amino acid identity to human and Xenopus Frizzled8, respectively. Like other family members, Frizzled8 has a complex expression pattern in the mouse embryo. It is expressed in the neural tube, brain, eye, somites, gut and bronchial arches.
Uniprot IDQ61091
Protein RefSeqNP_032084.2
mRNA RefSeqNM_008058.2

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