Mouse Anti-Human VEGFR-2/KDR
Slide this table
| Cat-Nr. | 101-M34 |
| Size | 100 µg |
| Price | 240 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#4(20I6)) |
| Isotype | IgG1 |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular KDR (110 kDa) as the immunizing antigen. Mouse IgG1 antibody (clone 4 (20I6)) from hybridomas was purified from cell culture supernatant by Protein G chromatography. |
| Antigen | recombinant human soluble extracellular KDR (D7) |
| Application | ELISA, WB, IHC, IF, FC |
| Synonyms | vascular endothelial growth factor receptor-2 ; KDR; FLK1; CD309; VEGF receptor 2; VEGFR2; kinase insert domain protein receptor |
| Description | VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. Human VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis. |
| Uniprot ID | P35968 |
| Protein RefSeq | NP_002244.1 |
| mRNA RefSeq | NM_002253.2 |
Figures
Up-regulation of VEGFR-2 in vein ECs of an intact human umbilical cord by bFGF. A fresh human umbilical cord was rinsed with PBS to remove residual blood cells, cut in small pieces (about 0.5 cm), incubated in EBM (1% FCS) and stimulated with or without 20 ng/ml bFGF for 24 h. Pieces were frozen in liquid nitrogen and used for immunohistochemistry using the mab anti-human VEGFR-2/Cl.4 (#101-M34) as detection antibody.
Bernhard Barleon et al., unpublished data
FACS analysis of VEGFR-2/KDR expression in HUVE cells [5µg/ml #101-M34; 5µg/ml PE goat anti-mouse IgG].
The experiment was performed by Trisha M. Westerhof, University of California, Irvine.
Up-regulation of VEGFR-2 in primary HUVECs by bFGF. Freshly isolated HUVECs (passage 1) were cultured in EBM. Subconfluent cultures were stimulated with VEGF (5 ng/ml) or bFGF (10 ng/ml) for 3 days. Total lysate was prepared and subjected to immunoprecipitation (anti-human VEGFR-2 [#101-M32] followed by Western blotting (anti-human VEGFR-2 [#101-M34].
Bernhard Barleon et. al., unpublished data
FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).
Western Analysis of anti-human esKDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions. Left panel: monoclonal antibody against the soluble KDR (D1-7); Right panel: polyclonal antibody (peptide) against the unique C-terminal end of esKDR.
Immunofluorescence staining (green) of VEGFR-2 (KDR) in primary human umbilical vein endothelial cells (HUVEC) with anti-human VEGFR-2 (Cl.4) (7,5µg/ml) [Cat# 101-M34] and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 (Dianova) was used 1:600.
Reference
- Similarities Between Stem Cell Niches in Glioblastoma and Bone Marrow: Rays of Hope for Novel Treatment Strategies. V.V.V. Hira et al., J Histochem Cytochem. 2020 Jan; 68(1): 33–57.
- Similarities and differences of human and experimental mouse lymphangiomas. Kasten P. et al., Dev Dyn. 2007 Oct;236(10):2952-61.
All prices plus VAT + possible delivery charges