Mouse Anti-Human E-Cadherin

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Cat-Nr.101-M132
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#21E9)
IsotypeIgG2
Species ReactivityHuman
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified recombinant human E-Cadherin. The IgG2 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Antigenrecombinant human E-Cadherin protein
ApplicationWB, IHC (P)
SynonymsCDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
DescriptionEpithelial (E)Cadherin (E-CAD), also known as Cadherin1, cell-CAM120/ 80 in the human, uvomorulin in the mouse, Arc1 in the dog, and LCAM in the chicken, is a member of the Cadherin family of cell adhesion molecules (gene name CDH1). Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, α, β, and γ (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a singlepass-transmembrane domain, and a short carboxyterminal cytoplasmic domain responsible for interacting with the catenins. E-Cadherin contains five extracellular calciumbinding domains of approx. 110 amino acids each (amino acids 155-697).
Uniprot IDP12830
Protein RefSeqNP_004351.1
mRNA RefSeqNM_004360.3

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