Mouse Anti-Human VEGF-A
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| Cat-Nr. | 101-M58 |
| Size | 100 µg |
| Price | 230 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#7G7) |
| Isotype | IgG1 |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | The monoclonal antibody was produced with the help of BALB/c mice using recombinant human VEGF165 (45 kDa) as the immunizing antigen. Mouse IgG1 antibody (clone 7G7) from hybridomas was purified from cell culture supernatant by Protein G chromatography. |
| Antigen | recombinant human VEGF165 (RT# 300-036) |
| Application | ELISA, WB, IHC |
| Synonyms | VEGFA; VPF; VEGF; MVCD1 |
| Description | Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34 - 42 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. Different isoforms can be generated by differential splicing (e.g. VEGF165). All eight cysteine residues involved in intra- and inter-chain disulfide bonds are conserved among these growth factors. A cDNA encoding a protein having a 53% amino acid sequence homology in the PDGF-like region of VEGF has been isolated from a human placental cDNA library. This protein, named placenta growth factor (PlGF), is now recognized to be a member of the VEGF family of growth factors. Two receptor tyrosine kinases have been described as putative VEGF receptors. Flt-1 (fms-like tyrosine kinase), and KDR (kinase-insert-domain-containing receptor) proteins have been shown to bind VEGF-A with high affinity. In vitro, VEGF is a potent endothelial cell mitogen. In cultured endothelial cells, VEGF can activate phospholipase C and induce rapid increases of free cytosolic Ca2+. VEGF has also been shown to be chemotactic for monocytes and osteoblasts. In vivo, VEGF can induce angiogenesis as well as increase microvascular permeability. As a vascular permeability factor, VEGF acts directly on the endothelium and does not degranulate mast cells. Based on its in vitro and in vivo properties, VEGF is expected to play important roles in inflammation and during normal and pathological angiogenesis, a process that is associated with wound healing, embryonic development, and growth and metastasis of solid tumors. |
| Uniprot ID | P15692 |
| Protein RefSeq | NP_001165097 |
| mRNA RefSeq | NM_001171626 |
Figures
Immunohistochemistry with a paraffin-bedded section of human Glioblastoma tissue.
Immunofluorescence staining (green) of VEGF-A in primary human umbilical vein endothelial cells (HUVEC) with anti-human VEGF-A #7G7 (7,5µg/ml) [Cat# 101-M58] and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 (Dianova) was used (1:600).
Immunofluorescence staining (green) of VEGF-A in a mixture of primary human dermal lymphatic endothelial cells (HDLEC) and normal human dermal fibroblasts (NHDF) with anti-human VEGF-A #7G7 (10µg/ml) [Cat# 101-M58] and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 (Dianova) was used (1:600).
VEGF-A Sandwich-ELISA using the monoclonal mouse antihuman VEGF-A antibody [Cat# 101-M58] as capture antibody and recombinant human VEGF189 [Cat# 300-094] as standard. A biotinylated monoclonal mouse anti-human VEGF-A antibody [Cat# 101-MBi60] was used for detection.
Western blot analysis of recombinant human VEGF165, mouse VEGF164 and human VEGF121 using a monoclonal mouse anti-human VEGF-A antibody [Cat# 101-M58]. There is no cross reactivity with mouse VEGF164.
Reference
- The MSC-MCF-7 Duet Playing Tumor Vasculogenesis and Angiogenesis onto the Chick Embryo Chorioallantoic Membrane. S. Comsa et al., In Vivo. 2020 Nov-Dec; 34(6): 3315–3325.
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