Rat Anti-Mouse ALK5

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Size100 µg
Price350 €
CategoryMonoclonal Antibody
Clone Nr.(#3A11)
Species ReactivityMouse
ReconstitutionCentrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse ALK-5 extracellular domain. IgG2 fraction of the culture supernatant was purified by Protein G affinity chromatography.
Antigenrecmobinant mouse ALK5 extracellular domain
ApplicationWB, IHC
SynonymsTgfbr1; ALK5; Alk-5; TbetaRI; AU017191; TbetaR-I
DescriptionMost cell types express three sizes of receptors for TGFβ. These are designated Type I (53 kDa), Type II (7085 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membranebound and soluble forms, binds TGFβ1, TGFβ2, and TGFβ3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane bound serine/threonine kinase that binds TGFβ1 and TGFβ3 with high affinity and TGFβ2 with a much lower affinity. The Type I receptor, originally known as ALK5 (Activin receptor-like kinase) is also a membranebound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGFβ. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.
Uniprot IDQ64729
Protein RefSeqNP_033396.1
mRNA RefSeqNM_009370.2

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