Rat Anti-Mouse BEC12

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Cat-Nr.103-M154
Size100 µg
Price275 €
CategoryMonoclonal Antibody
Clone Nr.(#LA5)
IsotypeIgG2a
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionCentrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Stability and StorageThe lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
PreparationThe antibody was produced by a rapid differential immunization of DA rats (licenced from Tokyo Women’s Medical University, Japan) with collagenase- and neuraminidase-treated mouse benign lymphangiomas. The rat IgG2a antibody was purified from cell culture supernatant by Protein G chromatography.
Antigenwith collagenase- and neuraminidase-treated mouse benign lymphangiomas
ApplicationWB, IHC, FC, IF
SynonymsBlood endothelial cell marker 12
DescriptionLA5 reacts with most mouse blood vessels except heart, large arteries and veins, but not with any lymphatic vessels. LA5 recognized a protein of around 12 kDa. The antigen recognized by LA5 was localized on both luminal and abluminal lymphatic endothelial cell membranes. Besides blood vessels, some lymphoid cells are also recognized.
Uniprot IDn.d.

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Reference

  1. Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels. J. Schniedermann et al., BMC Cell Biol. 2010; 11: 50.

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