Rat Anti-Mouse BEC12
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|Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
|Stability and Storage
|The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
|The antibody was produced by a rapid differential immunization of DA rats (licenced from Tokyo Women’s Medical University, Japan) with collagenase- and neuraminidase-treated mouse benign lymphangiomas. The rat IgG2a antibody was purified from cell culture supernatant by Protein G chromatography.
|with collagenase- and neuraminidase-treated mouse benign lymphangiomas
|WB, IHC, FC, IF
|Blood endothelial cell marker 12
|LA5 reacts with most mouse blood vessels except heart, large arteries and veins, but not with any lymphatic vessels. LA5 recognized a protein of around 12 kDa. The antigen recognized by LA5 was localized on both luminal and abluminal lymphatic endothelial cell membranes. Besides blood vessels, some lymphoid cells are also recognized.
- Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels. J. Schniedermann et al., BMC Cell Biol. 2010; 11: 50.
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