Mouse Anti-Human TIE-2
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|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml.|
|Stability and Storage||The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.|
|Preparation||The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular TIE-2 as the immunizing antigen. Mouse IgG1 antibody (#tek9) from hybridomas was purified from cell culture supernatant by Protein G chromatography.|
|Antigen||recombinant human soluble extracellular TIE-2|
|Application||ELISA, WB, FC|
|Synonyms||TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B; tek9; tek 9|
|Description||Tie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. Tie-2 is involved in vascular stabilization and remodeling. Although less well understood, Tie-1 may also act as an ANG receptor, possibly in complex with Tie-2. Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.|
- Angiopoietin-2 Stimulation of Endothelial Cells Induces αvβ3 Integrin Internalization and Degradation. M. Thomas et al., J Biol Chem. 2010 Jul 30; 285(31): 23842–23849.
- Identification of proangiogenic TIE2-expressing monocytes (TEMs) in human peripheral blood and cancer. Venneri MA et al., Blood. 2007 Jun 15;109(12):5276-85.
- Identification of a soluble form of the angiopoietin receptor TIE-2 released from endothelial cells and present in human blood. Reusch P. et al., Angiogenesis. 2001;4(2):123-31.
- Soluble Tie2 and Flt1 extracellular domains in serum of patients with renal cancer and response to antiangiogenic therapy. Harris AL et al., Clin Cancer Res. 2001 Jul;7(7):1992-7.
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