Mouse Anti-Human CCL-15
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|Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
|Stability and Storage
|Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
|This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of CCL-15 (also called MIP-1d).
|recombinant human CCL-15
|CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF1, human HCC1, and mouse MIP1γ, respectively. The gene for MIP1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocytederived dendritic cells. Human MIP1δ is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR1transfected cells.
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