Rat Anti-Mouse Follistatin-Related Gene Protein (FLRG)

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Cat-Nr.103-M222
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#7D11)
IsotypeIgG2
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant FLRG. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
Antigenrecombinant mouse FLRG
ApplicationWB
SynonymsFstl3; Flrg; E030038F23Rik
DescriptionFollistatin-Related Gene Protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Mouse FLRG cDNA encodes a 256 amino acid (aa) residue protein with a putative 23 aa signal peptide, an N-terminal domain, two cysteine rich follistatin-like domains (FS) and a C-terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin binding domain found within the first aminoterminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGFβ family ligands including activin, BMP2, 6, 7, and GDF8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGFβ and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293, and CHO cells.
Uniprot IDQ62356
Protein RefSeqNP_032073.2
mRNA RefSeqNM_008047.5

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