Mouse Anti-Human TIMP-1

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Cat-Nr.101-M123
Size100 µg
Price380 €
CategoryMonoclonal Antibody
Clone Nr.(#4A16)
IsotypeIgG2
Species ReactivityHuman
Formulationlyophilized
BufferPBS
ReconstitutionCentrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1). The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Antigenhuman recombinant TIMP-1 protein
ApplicationWB, IHC (P)
SynonymsTIMP1; EPA; EPO; HCI; CLGI; TIMP
DescriptionTIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.
Uniprot IDP01033
Protein RefSeqNP_003245
mRNA RefSeqNM_003254

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