Mouse Anti-Human VEGFR-1/Flt-1

Slide this table

Size50 µg
Price230 €
CategoryMonoclonal Antibody
Clone Nr.(#EWI)
Species ReactivityHuman, mouse
Stabilizer/CarrierBSA (50x), 0.02% sodium azide
ReconstitutionCentrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Stability and StorageThe lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
PreparationThe monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular Flt-1 (D1-D5) as the immunizing antigen. Mouse IgG1 antibody (#EWI) from hybridomas was purified from cell culture supernatant by Protein G chromatography. The antibody can be used for ELISA experiments, Western blotting, immunofluorescence, and immunoprecipitation. It will recognize recombinant and naturally occurring form of human VEGFR-1/Flt-1. The antibody cross-reacts with mouse Flt-1 (e.g. natural occurring soluble mouse Flt-1) in Western analysis.
Antigenrecombinant human soluble extracellular Flt-1 (D1-D5)
ApplicationELISA, WB, IP
Synonymsvascular endothelial growth factor receptor-1; FLT1; VEGF receptor 1;
DescriptionEndothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVEC supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.
Uniprot IDP17948
Protein RefSeqNP_001153392
mRNA RefSeqNM_001159920

All prices plus VAT + possible delivery charges