Rat Anti-Mouse E-Selectin

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Cat-Nr.103-M129
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#9J12)
IsotypeIgG2
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 400 µl sterile PBS and the final concentration is 500 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with cytokine stimulated-mouse endothelial membrane protein). IgG2 fraction of the culture supernatant was purified by Protein G affinity chromatography.
AntigenCytokine stimulated mouse endothelial protein
ApplicationIHC (P), FC
SynonymsSele; Elam; CD62E; E-selectin
DescriptionE-Selectin (Endothelial Leukocyte Adhesion Molecule1, ELAM1, CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL 1β and TNFα, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E-Selectin proteins share 81% amino acid similarity. E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.
Uniprot IDQ00690
Protein RefSeqNP_035475.1
mRNA RefSeqNM_011345.2

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