Mouse Anti-Human VEGFR-2/KDR
Slide this table
| Cat-Nr. | 101-M20 |
| Size | 100 µg |
| Price | 260 € |
| Category | Monoclonal Antibody |
| Clone Nr. | (#EIC) |
| Isotype | IgG1 |
| Species Reactivity | Human |
| Formulation | lyophilized |
| Buffer | PBS |
| Reconstitution | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. |
| Stability and Storage | The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots. |
| Preparation | The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular KDR (110 kDa) as the immunizing antigen. |
| Antigen | recombinant human soluble extracellular KDR (D7) |
| Application | ELISA, IHC, FC, WB |
| Synonyms | vascular endothelial growth factor receptor-2 ; KDR; FLK1; CD309; VEGF receptor 2; VEGFR2; kinase insert domain protein receptor |
| Description | VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. Human VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis. |
| Uniprot ID | P35968 |
| Protein RefSeq | NP_002244.1 |
| mRNA RefSeq | NM_002253.2 |
Figures
FACS analysis of VEGFR-2/KDR expression in HUVE cells (upper level) and EPCs derived from PBMcs (lower level) [5μg/ml #101-M20; 5μg/ml PE goat anti-mouse IgG].
The experiment was performed by Trisha M. Westerhof, University of California, Irvine.
Western Analysis of anti-human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions.
Functional ELISA with anti-human VEGFR-2/KDR [Cat# 101-M20]. Recombinant human soluble KDR (D7) [Cat# S01-002] was coated with increasing amounts in a 96 well microtiter plate. The antibody was used at 1μg/ml.
Reference
- Multicolor Immunofluorescent Imaging of Complex Cellular Mixtures on Micropallet Arrays Enables the Identification of Single Cells of Defined Phenotype. T. M. Westerhof et al., Adv Healthc Mater. 2016 Apr 6; 5(7): 767–771.
- Only a specific subset of human peripheral-blood monocytes has endothelial-like functional capacity. Elzafir Elsheikh et al., Blood 2005 106:2347-2355.
- Expression of Vascular Endothelial Growth Factor Receptor-2 or Tie-2 on Peripheral Blood Cells Defines Functionally Competent Cell Populations Capable of Reendothelialization. Grzegorz Nowak et al., Circulation. 2004 Dec 14;110(24):3699-707.
All prices plus VAT + possible delivery charges