Rat Anti-Mouse 4-1BB receptor

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Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#4G33)
Species ReactivityMouse
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant 4-1BB extracellular domain. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
Antigenrecombinant 4-1BB Extracellular domain
ApplicationWB, FC
SynonymsTnfrsf9; ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
Description4-1BB, also known as CD137 and ILA (induced by lymphocyte activation), is a TNF receptor superfamily member and has been designated TNFRSF9. Mouse 4-1BB cDNA encodes a 256 amino acid (aa) residues type I transmembrane protein with a putative 23 aa signal peptide, a 164 aa extracellular domain, a 21 aa transmembrane domain and a 48 aa cytoplasmic region. A soluble 4-1BB is released from surfaces of cells expressing the transmembrane protein. Mouse4-1BB shares approximately 60% aa sequence identity with its human counterpart. 41BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils. The ligand for 4-1BB (4-1BBL), also named TNFSF9, belongs to the TNF ligand superfamily. 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines. In response to 4-1BBL binding, 4-1BB transduce a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In vivo, the costimulatory activity of 4-1BB has been shown to be important in graftv shost disease and antiviral CTL responses. On dendritic cells, 4-1BB is a DCactivating molecules that enhances cytokine production and upregulates expression of B71 and B72 costimulatory molecules, resulting in an improved ability to stimulate T cell responses.
Uniprot IDP20334
Protein RefSeqNP_035742.1
mRNA RefSeqNM_011612.2

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