Rat Anti-Mouse MAdCAM-1

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Cat-Nr.103-M432
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#11A55)
IsotypeIgG2
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of MAdCAM-1 EC domain.
Antigenrecombinant mouse MAdCAM-1 EC domain
ApplicationWB, Blocking
SynonymsMadcam1; AV211525
DescriptionMucosal addressin cell adhesion molecule1 (MAdCAM1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinuslining cells of the spleen. The integrin, α4β7, has been shown to function as the MAdCAM1 receptor. The Ig domains of MAdCAM1 have been found to be critical to α4β7 binding. The mucin domain has been shown to have activity in LSelectin binding. MAdCAM1 expression has been demonstrated to be upregulated by TNFα and IL1β. MAdCAM1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly upregulated in IBD and most likely serves to recruit α4β7expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM1/ α4β7 interaction plays a role in diabetes development in this model. Mouse MAdCAM1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.
Uniprot IDG5E838
Protein RefSeqNP_038619.2
mRNA RefSeqNM_013591.2

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