Rat Anti-Mouse T-cell Immunoglobulin Mucin-3 (TIM-3)

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Cat-Nr.103-M276
Size100 µg
Price250 €
CategoryMonoclonal Antibody
Clone Nr.(#6F46)
IsotypeIgG2
Species ReactivityMouse
Formulationlyophilized
BufferPBS
ReconstitutionReconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
Stability and StorageLyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.
PreparationThis antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant TIM-3. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography.
Antigenmouse recombinant protein of TIM-3
ApplicationWB
SynonymsHavcr2; Tim3; TIM-3; Timd3
DescriptionT cell immunoglobulin mucin-3 (TIM-3) is a member of the T-cell Immunoglobulin- and Mucin-domain-containing family of type I membrane glycoproteins that regulate autoimmune and allergic disease. TIM-3 is selectively expressed on Th1 cells and interacts with galectin-9. It negatively regulates Th1 responses and affects macrophage activation. The 280 aa mature human TIM-3 contains a V-type Ig-like domain that shows multiple polymorphisms, followed by a mucin-like domain in the 171 aa extracellular region, which shares 60% amino acid identity with mouse TIM-3 ECD. One splice variant of TIM-3 is truncated within the mucin domain and presumably is secreted.
Uniprot IDQ8VIM0
Protein RefSeqNP_599011.2
mRNA RefSeqNM_134250.2

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