Mouse Anti-Human Podoplanin
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|Reconstitution||Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of > 0.5 mg/ml|
|Stability and Storage||The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.|
|Preparation||The monoclonal antibody was produced with the help of BALB/c mice using MDCK cells stably expressing gp36 (Podoplanin) protein as the immunizing antigen. The mouse IgG1 antibody (#18H5) from hybridomas was purified from cell culture supernatant by Protein G chromatography.|
|Antigen||gp36 (Podoplanin)-expressing MDCK cells|
|Application||WB, IHC, IF, FC|
|Synonyms||PDPN; T1A; GP36; GP40; Gp38; OTS8; T1A-2; AGGRUS; HT1A-1; PA2.26|
|Description||Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.|
- Heterogeneity of stromal cells in the human splenic white pulp. Fibroblastic reticulum cells, follicular dendritic cells and a third superficial stromal cell type. Steiniger B. et al., Immunology. 2014 Nov; 143(3): 462–477.
- Lack of Evidence for the Direct Activation of Endothelial Cells by Adult Female and Microfilarial Excretory-Secretory Products. T. Weinkopff and P. Lammie, PLoS One. 2011; 6(8): e22282.
- Aberrant lymphatic development in euploid fetuses with increased nuchal translucency including Noonan syndrome. de Mooij YM et al., Prenat Diagn. 2011 Feb;31(2):159-66.
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