Porcine TNF-alpha

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Cat-Nr.P40-001
Size20 µg
Price180 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 95% by SDS-PAGE & RP-HPLC
Length [aa]157
Molecular Weight17.2 kDa
Biological ActivityThe ED50 as determined by the cytotoxicity of porcine PK15 cells was found to be  0.001-0.015 ng/ml.
SynonymsTNF; TNFa
DescriptionTumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders.
Protein SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Ser-Ser.
Uniprot IDP23563
Protein RefSeqNP_999187.1
mRNA RefSeqNM_214022.1

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