Human IL-10

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Cat-Nr.100-049
Size10 µg
Price170 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE & HPLC analyses
Length [aa]161
Molecular Weight18.6 kDa
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityDetermined by the dose-dependent co-stimulation (with human IL-4) of MC/9 cells. The ED50 was found to be ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
Species ReactivityMonkey, Mouse, Human, Bacteria, Virus
Buffer5mM Sodium Phosphate, pH 7.2
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in 5mM Sodium Phosphate, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Stability and StorageThe lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.
SynonymsIL10; CSIF; TGIF; GVHDS; IL-10; IL10A
DescriptionInterleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.5 kDa protein that shares over 80% homology with the Epstein-Barr virus protein BCRFI. The reported biological activities of IL-10, which may be inter-related, include inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hyper-sensitivity response, and stimulation of the TH2 cell response which results in elevated antibody production.
Protein SequenceMSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
Uniprot IDP22301
Protein RefSeqNP_000563.1
mRNA RefSeqNM_000572

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