Human IFN-beta

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Cat-Nr.100-191
Size20 µg
Price170 €
SourceCHO cells
Formulationlyophilized
Purity Confirmation> 95% by SDS-PAGE & HPLC analyses
Length [aa]166
Molecular Weight20 kDa
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityAssay #1: Measured by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 20-30 ng/ml. Assay #2: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml (≥ 1 x 107 units/mg).
Species ReactivityMonkey, Mouse, Human
SynonymsIFNB1; IFB; IFF; IFNB;
DescriptionProteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFN-alpha is an acid stable molecule that signals through IFN-alpha/betaR, which is also used by IFN-beta. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. Recombinant human IFN-beta is a 20.0 kDa protein containing 166 amino acid residues. Due to glycosylation, IFN-beta has an approximate MW of 22.3 kDa based on SDS-PAGE gel and Mass Spectrometry.
Protein SequenceMSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN
Uniprot IDP01574
Protein RefSeqNP_002167.1
mRNA RefSeqNM_002176

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