Human RANKL, soluble

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Cat-Nr.100-156
Size10 µg
Price170 €
SourceCHO cells
LabelHis-Tag
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE & HPLC analyses
Length [aa]198
Molecular Weight22.1 kDa
Endotoxin Levels< 0.1 ng /mg of protein (<1EU/mg).
Biological ActivityDetermined by its ability to induce NF-κB in RAW264.7 cells in the absence of any cross-linking.
Species ReactivityHuman
ReconstitutionCentrifuge the vial prior to opening!
Synonymssoluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-Related Activation-induced Cytokine), OPGL, ODF (Osteoclast Differentiation Factor), CD254
DescriptionRANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction,promote the survival of RANK + T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells,including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Human RANKL is reactive on murine cells. Recombinant Human sRANK Ligand is a 22.1 kDa polypeptide comprised of the TNF-homologous region of RANKL and a N-terminal His-tag.
Protein SequencesHHHHHHHHP SPGGSGGSQH IRAEKAMVDG SWLDLAKRSK LEAQPFAHLT INATDIPSGS HKVSLSSWYH DRGWGKISNM TFSNGKLIVN QDGFYYLYAN ICFRHHETSG DLATEYLQLM VYVTKTSIKI PSSHTLMKGG STKYWSGNSE FHFYSINVGG FFKLRSGEEI SIEVSNPSLL DPDQDATYFG AFKVRDID
Uniprot IDO14788
Protein RefSeqNP_003692.1
mRNA RefSeqNM_003701.3

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