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Human FGF-2 (basic)

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Cat-Nr.300-002
Size25 µg
Price110 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE
Length [aa]153
Molecular Weight17.0 kDa
N Terminal SequenceAGSITTL
Endotoxin Levels< 0.1 ng per µg of human FGF-2
Biological ActivityThe ED50 for stimulation of cell proliferation in HUVECs by human FGF-2 (basic) has been determined to be in the range of 0.1-2 ng/ml. The WHO standard #90/712 was used as control.
Species ReactivityHuman, Mouse
BufferPBS
ReconstitutionThe lyophilized FGF-2 (basic) should be reconstituted in water to a concentration not lower than 50 µg/ml. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin.
Stability and StorageLyophilized samples are stable for greater than six months at -20°C to -70°C.
SynonymsFGF2; BFGF; FGFB; HBGF-2; basic Fibroblast growth factor (bFGF); Heparin binding growth factor-2
DescriptionFGF basic (FGF2, HBGF2) is one of at least 23 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 17 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted. Transcription from alternate start sites produces 21-24 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH3T3 cells. The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine and sheep FGF basic. Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions.
Protein SequenceAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Uniprot IDP09038
Protein RefSeqNP_001997.5
mRNA RefSeqNM_002006.4

Figures

Figure 1.
SDS-PAGE analysis of recombinant human FGF-2 (basic). Samples were loaded in 15% SDS-polyacrylamide gel under reducing and non-reducing condition and stained with Coomassie blue.
Figure 2.
FGF2-induced proliferation of primary normal human dermal fibroblasts. NHDF cells were stimulated with increasing amounts of human FGF-2 (basic).
Figure 3.
FGF2-induced proliferation of primary human umbilical vein endothelial cells (HUVEC). The cells were stimulated using recombinant human FGF-2 and the WHO standard 90/712. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.
Figure 4.
FGF2-induced proliferation of primary human dermal lymphatic endothelial cells. HDLECs were stimulated with increasing amounts of human FGF-2 (basic).

Reference

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