Human FGF-2 (basic)

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Cat-Nr.300-002
Size25 µg
Price100 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE & silver stain
Length [aa]153
Molecular Weight17.0 kDa
N Terminal SequenceAGSITTL
Endotoxin Levels< 0.1 ng per µg of human FGF-2
Biological ActivityThe ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells by human FGF-2 (basic) has been determined to be in the range of 0.1-2 ng/ml.
Species ReactivityHuman, Mouse
BufferPBS
ReconstitutionThe lyophilized FGF-2 (basic) should be reconstituted in water to a concentration not lower than 50 µg/ml. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin.
Stability and StorageLyophilized samples are stable for greater than six months at -20 °C to -70 °C.
SynonymsFGF2; BFGF; FGFB; HBGF-2; basic Fibroblast growth factor (bFGF); Heparin binding growth factor-2
DescriptionFGF basic (FGF2, HBGF2) is one of at least 23 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 17 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted. Transcription from alternate start sites produces 21-24 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH3T3 cells. The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine and sheep FGF basic. Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions.
Protein SequenceAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Uniprot IDP09038
Protein RefSeqNP_001997.5
mRNA RefSeqNM_002006.4

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Reference

  1. Blood Outgrowth and Proliferation of Endothelial Colony Forming Cells are Related to Markers of Disease Severity in Patients with Pulmonary Arterial Hypertension. Smits J. et al., Int J Mol Sci. 2018 Nov 27;19(12). pii: E3763.
  2. A local uPAR-plasmin-TGFβ1 positive feedback loop in a qualitative computational model of angiogenic sprouting explains the in vitro effect of fibrinogen variants. Boas S.E.M. et al., PLoS Comput Biol. 2018 Jul 6;14(7):e1006239.
  3. Chronophin regulates active vitamin B6 levels and transcriptomic features of glioblastoma cell lines cultured under non-adherent, serum-free conditions. Schulze M. et al., BMC Cancer. 2018 May 3;18(1):524.
  4. Pericyte-expressed Tie2 controls angiogenesis and vessel maturation. M. Teichert et al., Nat Commun. 2017; 8: 16106.
  5. Burn Eschar Stimulates Fibroblast and Adipose Mesenchymal Stromal Cell Proliferation and Migration but Inhibits Endothelial Cell Sprouting. H. N. Monsuur et al., Int J Mol Sci. 2017 Aug; 18(8): 1790.
  6. Isocitrate dehydrogenase mutations suppress STAT1 and CD8+ T cell accumulation in gliomas. G. Kohanbash et al., J Clin Invest. 2017 Apr 3; 127(4): 1425–1437.
  7. CD34 expression modulates tube-forming capacity and barrier properties of peripheral blood-derived endothelial colony-forming cells (ECFCs). D. Tasev et al., Angiogenesis. 2016; 19: 325–338.
  8. Diagnostic and clinical relevance of the autophago-lysosomal network in human gliomas. L. Jennewein et al., Oncotarget. 2016 Apr 12; 7(15): 20016–20032.
  9. Molecular dissection of the valproic acid effects on glioma cells. S. Hoja et al., Oncotarget. 2016 Sep 27; 7(39): 62989–63002.
  10. Extensive Characterization and Comparison of Endothelial Cells Derived from Dermis and Adipose Tissue: Potential Use in Tissue Engineering. H. N. Monsuur et al., PLoS One. 2016; 11(11): e0167056.
  11. Endothelial Cells Derived from Non-malignant Tissues Are of Limited Value as Models for Brain Tumor Vasculature. Lohr J. et al., Anticancer Res. 2015 May;35(5):2681-90.
  12. CD95 promotes metastatic spread via Sck in pancreatic ductal adenocarcinoma. M. Teodorczyk et al., Cell Death Differ. 2015 Jul; 22(7): 1192–1202.
  13. Inhibition of Notch1 signaling overcomes resistance to the death ligand Trail by specificity protein 1-dependent upregulation of death receptor 5. A. Fassl et al., Cell Death Dis. 2015 Oct; 6(10): e1921.
  14. Common and specific effects of TIE2 mutations causing venous malformations. M. Nätynki et al., Hum Mol Genet. 2015 Nov 15; 24(22): 6374–6389.
  15. Dual targeting of glioblastoma with chimeric antigen receptor-engineered natural killer cells overcomes heterogeneity of target antigen expression and enhances antitumor activity and survival. S. Genßler et al., Oncoimmunology. 2016 Apr; 5(4): e1119354.
  16. Immunohistochemical Assessment of Phosphorylated mTORC1-Pathway Proteins in Human Brain Tumors. P. N. Harter et al., PLoS One. 2015; 10(5): e0127123.
  17. Long-Term Expansion in Platelet Lysate Increases Growth of Peripheral Blood-Derived Endothelial-Colony Forming Cells and Their Growth Factor-Induced Sprouting Capacity. D. Tasev et al., PLoS One. 2015; 10(6): e0129935.
  18. Systemic miRNA-7 delivery inhibits tumor angiogenesis and growth in murine xenograft glioblastoma. N. Babae et al., Oncotarget. 2014 Aug; 5(16): 6687–6700.
  19. Hypoxia Enhances the Antiglioma Cytotoxicity of B10, a Glycosylated Derivative of Betulinic Acid. S. Fischer et al., PLoS One. 2014; 9(4): e94921.
  20. New procedure for epidermal cell isolation using kiwi fruit actinidin, and improved culture of melanocytes in the presence of leukaemia inhibitory factor and forskolin. Yarani R. et al., Cell Prolif. 2013 Jun;46(3):348-55.
  21. Endothelial Heparan Sulfate 6-O-Sulfation Levels Regulate Angiogenic Responses of Endothelial Cells to Fibroblast Growth Factor 2 and Vascular Endothelial Growth Factor. C. Ferreras et al., J Biol Chem. 2012 Oct 19; 287(43): 36132–36146.
  22. Tumor angiogenesis is enforced by autocrine regulation of high-mobility group box 1. van Beijnum JR et al., Oncogene. 2013 Jan 17;32(3):363-74.
  23. Loss of Akt activity increases circulating soluble endoglin release in preeclampsia: identification of inter-dependency between Akt-1 and heme oxygenase-1. M. J. Cudmore et al., Eur Heart J. 2012 May; 33(9): 1150–1158.
  24. Interactions of Human Endothelial and Multipotent Mesenchymal Stem Cells in Cocultures. C. Ern et al., Open Biomed Eng J. 2010; 4: 190–198.
  25. Spheroid-based human endothelial cell microvessel formation in vivo. A.M. Laib et al., Nat Protoc. 2009;4(8):1202-15.
  26. Degradation of soluble VEGF receptor-1 by MMP-7 allows VEGF access to endothelial cells. T.K. Ito et al., Blood. 2009 Mar 5;113(10):2363-9.

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