Human IFN-gamma

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Cat-Nr.100-039
Size100 µg
Price170 €
SourceE. coli
Formulationlyophilized
Purity Confirmation> 98% by SDS-PAGE & HPLC analyses
Length [aa]144
Molecular Weight16.8 kDa
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityAssay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml. Assay #2: The ED50 was determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml (> 2 x 107 units/mg).
Species ReactivityMonkey, Human, Mouse, Leech, Pig,
Buffer10mM Sodium Phosphate, pH 7.4
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to ‑80°C.
Stability and StorageThe lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.
SynonymsIFNG; IFG; IFI;
DescriptionInterferon-gamma (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Recombinant Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.
Protein SequenceMQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ
Uniprot IDP01579
Protein RefSeqNP_000610.2
mRNA RefSeqNM_000619

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