Human TPO (Animal Free)

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Cat-Nr.100-109-AF
Size10 µg
Price230 €
SourceE. coli
Formulationlyophilized
Purity Confirmation≥ 98% by SDS-PAGE gel and HPLC analyses.
Length [aa]174
Molecular Weight18.6 kDa
Endotoxin Levels< 0.01 ng/ug of protein (< 0.1 EU/ug)
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Species ReactivityHuman, Human + Hamster, Monkey, Mouse, Rat
SynonymsThrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF
DescriptionTPO is a lineage-specific growth factor produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor, and acts as an important regulator of circulating platelets. Human and murine TPO exhibit cross-species reactivity. The human TPO gene encodes for a 353 amino acid glycoprotein, which contains a 21 amino acid signal sequence, a 15 amino acid erythropoietin-like domain, and a highly glycosylated 179 amino acid C-terminal domain. Recombinant Human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein.
Protein SequenceSPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL
Uniprot IDP40225
Protein RefSeqNP_000451.1
mRNA RefSeqNM_000460.2

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