Human SMCY/JARID1D

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Cat-Nr.300-064
Size25 µg
Price250 €
SourceE. coli
LabelHis-Tag
Formulationlyophilized
Purity Confirmation> 95% by SDS-PAGE & visualized by silver stain
Length [aa]301
Molecular Weight33.87 kDa
N Terminal SequenceALLVALQRLPVRLP
Biological ActivityTesting in progress.
Species ReactivityHuman
Buffer50 mM NaP, 300 mM NaCl, pH 8.0
ReconstitutionCentrifuge vial prior to opening. The lyophilized human SMCY is supplied in lyophilized form and can be reconstituted in PBS or water. This solution can be diluted into other buffered solutions or stored frozen for future use.
Stability and StorageThe lyophilized human SMCY, though stable at room temperature, is best stored in working aliquots at -20 °C to -70 °C.
SynonymsKDM5D lysine (K)-specific demethylase 5D, HY; HYA
DescriptionH-Y antigen is defined as a male histocompatibility antigen that causes rejection of male skin grafts by female recipients of the same inbred strain of rodents. Male-specific, or H-Y antigen(s), are also detected by cytotoxic T cells and antibodies. H-Y antigen appears to be an integral part of the membrane of most male cells. In addition, H-Y antibodies detect a soluble form of H-Y that is secreted by the testis. The gene (Smcy/SMCY) coding for H-Y antigen detected by T cells has been cloned. It is expressed ubiquitously in male mice and humans, and encodes an epitope that triggers a specific T-cell response in vitro. Additional epitopes coded for by different Y-chromosomal genes are probably required in vivo for the rejection of male grafts by female hosts. The molecular nature of H-Y antigen detected by antibodies on most male cells is not yet known. Testis-secreted, soluble H-Y antigen, however, was found to be identical to Müllerian-inhibiting substance (MIS). MIS cross-reacts with H-Y antibodies and identical findings were obtained for soluble H-Y antigen and MIS, i.e., secretion by testicular Sertoli and, to a lesser degree, ovarian cells, binding to a gonad-specific receptor, induction of gonadal sex reversal in vitro and, in cattle, in vivo. H-Y antisera also detect a molecule or molecules associated with the heterogametic sex in non-mammalian vertebrates. Molecular data on this antigen or antigens are not yet available.
Protein SequenceALLVALQRLPVRLPEGEALQCLTERAIGWQDRARKALASEDVTALLRQLAELRQQLQAKPRPEEASVYTSATACDPIREGSGNNISKVQGLLENGDSVTSPENMAPGKGSDLELLSSLLPQLTGPVLELPEAIRAPLEELMMEGDLLEVTLDENHSIWQLLQAGQPPDLDRIRTLLELEKFEHQGSRTRSRALERRRRRQKVDQGRNVENLVQQELQSKRARSSGIMSQVGREEEHYQEKADRENMFLTPSTDHS
Uniprot IDQ9BY66
Protein RefSeqNP_001140177.1
mRNA RefSeqNM_001146705.1

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