Human GDNF (Animal Free)

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Cat-Nr.100-030S-AF
Size2 µg
Price135 €
SourceE. coli
Formulationlyophilized
Purity Confirmation≥ 98% by SDS-PAGE gel and HPLC analyses.
Length [aa]135
Molecular Weight15 kDa
Endotoxin Levels< 0.01 ng/ug of protein (< 0.1 EU/ug)
Biological ActivityDetermined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml.
Species ReactivityHuman, Monkey, Mouse
SynonymsGDNF; ATF1; ATF2; HSCR3; HFB1-GDNF
DescriptionGDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin.  These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures.  GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors.  GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons.  Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability.  The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers.  Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa.
Protein SequenceMSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Uniprot IDP39905
Protein RefSeqNP_000505.1
mRNA RefSeqNM_000514

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