Human TWEAK (Animal Free)

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Cat-Nr.100-111S-AF
Size5 µg
Price135 €
SourceE. coli
Formulationlyophilized
Purity Confirmation≥ 98% by SDS-PAGE gel and HPLC analyses.
Length [aa]154
Molecular Weight17 kDa
Endotoxin Levels< 0.01 ng/ug of protein (< 0.1 EU/ug)
Biological ActivityAssay #1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10 ng/ml. Assay #2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-γ. The ED50 for this effect is between 30-45 ng/ml.
Species ReactivityMouse, Human
SynonymsTNFSF12; APO3L; DR3LG; TWEAK
DescriptionTWEAK belongs to the TNF family of ligands, and signals through TWEAKR, also known as TNFRSF12A. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK has the ability to induce NF-κB activation and chemokine secretion, and to exert an apoptotic activity in certain cells, such as HT-29 human adenocarcinoma cells when cultured in the presence of IFN-γ. TWEAK also promotes proliferation and migration of endothelial cells. The human TWEAK gene encodes for a 249 amino acid type II transmembrane protein, which contains a 21 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 207 amino acid extracellular domain. Recombinant Human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF-homologous region of TWEAK, and is generated by proteolytic processing of the full length membrane-anchored TWEAK protein.
Protein SequenceMKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH
Uniprot IDO43508
Protein RefSeqNP_003800.1
mRNA RefSeqNM_003809.2

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