Chicken Growth Hormone mutant (G119R)

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Size50 µg
Price295 €
SourceE. coli
Purity Confirmation> 98.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.
Length [aa]191
Molecular Weight22.3 kDa
N Terminal SequenceATFPA
Endotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)
Biological ActivityChicken recombinant growth hormone G119R mutant did not bind to ovine growth hormone extracellular domain (ECD) and was devoid of any biological activity in FDC-P1 3B9 cells. However, in binding experiments that were carried out using chicken liver membranes, both ovine growth hormone and chicken recombinant growth hormone showed similar IC50 values in competition with 125I-oGH, while the IC50 of chicken recombinant chicken growth hormone G119R mutein was 10-fold higher. These results emphasize the importance of species specificity and indicate the possibility of antagonistic activity of chicken recombinant growth hormone G119R in homologous system.
ReconstitutionIt is recommended to reconstitute the lyophilized chicken recombinant growth hormone in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
SynonymsSomatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone
DescriptionChicken recombinant growth hormone (chGH) mutein G119R produced in E.Coli (22 K) is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22.3 kDa. For reference see Eliasiewicz et al. (2006) Ann N Y Acad Sci. 1091:501-508.
Uniprot IDP08998
Protein RefSeqNP_989690.1
mRNA RefSeqNM_204359.2

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