Mouse M-CSF
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| Cat-Nr. | M10-078 |
| Size | 10 µg |
| Price | 190 € |
| Source | E. coli |
| Formulation | lyophilized |
| Purity Confirmation | > 98% by SDS-PAGE |
| Length [aa] | 156 |
| Molecular Weight | 36.4 kDa |
| N Terminal Sequence | MKEVS |
| Biological Activity | The biological activity was determined by the induction of proliferation in the NFS 60 cell line. |
| Species Reactivity | Mouse, Human, Rat |
| Buffer | PBS |
| Reconstitution | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C. |
| Synonyms | Csf1; op; Csfm; MCSF; C87615 |
| Description | M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant murine M-CSF is a 36.4 kDa homodimeric protein containing two 156 amino acid polypeptide subunits. |
| Protein Sequence | MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP |
| Uniprot ID | P07141 |
| Protein RefSeq | NP_031804.3 |
| mRNA RefSeq | NM_007778 |
Figures
SDS-PAGE analysis of recombinant mouse M-CSF. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.
Proliferation assay with NFS60 cells. The cells were stimulated with increasing amounts of recombinant mouse M-CSF.
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